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sy 5609  (MedChemExpress)


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    Structured Review

    MedChemExpress sy 5609
    Sy 5609, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sy+5609/SY-5609/10__1158_slash_1535___7163__mct___25___1529-49-10-33
    Average 94 stars, based on 9 article reviews
    sy 5609 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: CDK7 is a novel therapeutic target in fibrolamellar carcinoma
    Article Snippet: Approximately 2.0 × 10 4 purified PHH cells/well were seeded in rat tail collagen (collagen type I) (Life Technologies, Carlsbad, CA) coated 96-well plate and incubated in hepatocyte Incubation medium at 37 °C and 5% CO 2 . .. Twenty-four hours after incubation, PHHs were treated with SY-5609 (100 pM - 10 μM) (MedChemExpress, Monmouth Junction, NJ) or vehicle (DMSO) for 2-5 days. .. Cell viability was detected using CellTiter Glo 2 reagent (Promega, Madison, WI).

    Article Title: CDK7 is a novel therapeutic target in fibrolamellar carcinoma
    Article Snippet: .. To investigate the synergistic effect between SY-5609 (CDK7 inhibitor) and VIP-152 or NVP-2 (CDK9 inhibitors, MedChemExpress, Monmouth Junction, NJ), HepG2 DNAJB1-PRKACA (H33) cells (2.0 × 10 3 cells/well) were seeded in an 8x8 matrix in a 96-well plate and incubated with RPMI1640 cell culture medium for 24 hours at 37 °C and 5% CO 2 . ..

    Article Title: CDK7 is a novel therapeutic target in fibrolamellar carcinoma
    Article Snippet: To measure the apoptosis activity in HepG2 cells and HepG2 DNAJB1-PRKACA clones, we used Caspase-Glo 3/7 and 8 assay systems (Promega, Madison, WI). .. Approximately 2.0 × 10 3 cells/well were seeded in a 96-well plate and (1) incubated in RPMI1640 culture medium over 4 days at 37 °C and 5% CO 2 or (2) incubated in RPMI1640 culture medium containing SY-5609 (0.1-10 μM) (MedChemExpress, Monmouth Junction, NJ) or DMSO for 24 hours. .. After addition of the Caspase-Glo 3/7 or 8 reagent to cell culture medium in 96 well plate and incubation for 30 minutes, luminescence was measured by CLARIOstar Plus microplate reader (BMG Labtech, Cary, NC).

    Article Title: CDK7 is a novel therapeutic target in fibrolamellar carcinoma
    Article Snippet: .. HepG2 and HepG2 DNAJB1-PRKACA clones were incubated with RPMI1640 cell culture for 24 hours, treated with SY-5609 (1-300 nM) (MedChemExpress, Monmouth Junction, NJ), YKL-5-124 (1-300 nM) (MedChemExpress, Monmouth Junction, NJ) or vehicle (DMSO) for 24 hours. .. Total RNA was isolated from cells using the Qiagen RNeasy kit (Qiagen, Hilden, Germany).

    Article Title: CDK7 is a novel therapeutic target in fibrolamellar carcinoma
    Article Snippet: .. After incubation, cells were treated with SY-5609 (1 pM-10 μM) (MedChemExpress, Monmouth Junction, NJ), YKL-5-124 (100 pM-10 μM) (MedChemExpress, Monmouth Junction, NJ) or vehicle (DMSO) for 48, 72 hours or 120 hours. ..

    Cell Culture:

    Article Title: CDK7 is a novel therapeutic target in fibrolamellar carcinoma
    Article Snippet: .. To investigate the synergistic effect between SY-5609 (CDK7 inhibitor) and VIP-152 or NVP-2 (CDK9 inhibitors, MedChemExpress, Monmouth Junction, NJ), HepG2 DNAJB1-PRKACA (H33) cells (2.0 × 10 3 cells/well) were seeded in an 8x8 matrix in a 96-well plate and incubated with RPMI1640 cell culture medium for 24 hours at 37 °C and 5% CO 2 . ..

    Article Title: CDK7 is a novel therapeutic target in fibrolamellar carcinoma
    Article Snippet: .. HepG2 and HepG2 DNAJB1-PRKACA clones were incubated with RPMI1640 cell culture for 24 hours, treated with SY-5609 (1-300 nM) (MedChemExpress, Monmouth Junction, NJ), YKL-5-124 (1-300 nM) (MedChemExpress, Monmouth Junction, NJ) or vehicle (DMSO) for 24 hours. .. Total RNA was isolated from cells using the Qiagen RNeasy kit (Qiagen, Hilden, Germany).

    Clone Assay:

    Article Title: CDK7 is a novel therapeutic target in fibrolamellar carcinoma
    Article Snippet: .. HepG2 and HepG2 DNAJB1-PRKACA clones were incubated with RPMI1640 cell culture for 24 hours, treated with SY-5609 (1-300 nM) (MedChemExpress, Monmouth Junction, NJ), YKL-5-124 (1-300 nM) (MedChemExpress, Monmouth Junction, NJ) or vehicle (DMSO) for 24 hours. .. Total RNA was isolated from cells using the Qiagen RNeasy kit (Qiagen, Hilden, Germany).

    other:

    Article Title: Sensitivity of pancreatic adenocarcinoma cells to blockade of post-initiation CDK-dependent Pol II transcription cycle checkpoints
    Article Snippet: CCT-251921 (HY-19984, (59)), OTS964 (HY-160 12467, (60)), THZ531 (HY-103618, (61)), SY-5609 (HY-138293, (62)), CR8 (HY-18340, (63)), 161 Trametinib (HY-10999), Binimetinib (HY-15202), Sotorasib (HY-114277), Adagrasib (HY-162 130149), and MRTX1133 (HY-164813, (14)) were purchased from MedChemExpress 163 [RRID:SCR_025062].



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